Anticholinesterase Properties of Mareya Micrantha (Benth) Mull. Arg. (Euphorbiaceae) and its Fractions on the Cholinesterase Model of Isolated Rabbit Duodenum

Abstract

Abstract

The study of the anticholinesterase properties of Mareya micrantha, a plant used in African pharmacopoeia as a laxative, was carried out on cholinesterases of the rabbit duodenum. At 0.15 mg / ml, the aqueous extract (MAR), its fraction (F5) and its sub-fraction (F52), depending on their state of purity, decrease the catalytic speed of cholinesterases during the hydrolysis of the acetylthiocholine (ASCh), without significantly influencing the Michaelis constant. The sub-fraction (F52) with the best anti-cholinesterase and myostimulant effects revealed by the bio-guided study is 25% more effective than MAR. A study of the High performance liquid chromatography (HPLC) chromatogram of this sub-fraction F52 shows that this plant is rich in phenolic molecules.